Journal: bioRxiv

Article Title: STMN2 protein depletion via translation deficits and stress granules and its compensation in ALS

doi: 10.64898/2026.01.25.701321

Figure Lengend Snippet: a Stress paradigms used in the study for SH-SY5Y cells and human motor neurons. Schematic was prepared using BioRender. b,c Chronic but not repetitive oxidative stress downregulates STMN2 protein. Representative western blot and quantification (b) and representative immunostaining images (c) are shown for SH-SY5Y cells. N=3; *p<0.05, Mann-Whitney U test. Scale bar 15 µm. d Chronic or repetitive stress do not cause visible changes to TDP-43 subcellular distribution or its nuclear condensation. Representative images for SH-SY5Y cells are shown. Scale bar, 10 µm. e Chronic stress leads to a reduction in global protein translation. Translation efficiency was analysed by ribopuromycilation in SH-SY5Y cells after applying the chronic stress paradigm as shown in a. Representative western blot is shown. f STMN2 is a short-lived protein, which is more stable in postmitotic as compared to proliferative neuronal cells, as demonstrated by cycloheximide (CHX) pulse-chase. Rocaglamide-A and sylvestrol were included as additional controls – translational inhibitors (30-min treatment). Representative western blots and quantification by densitometry are shown. N=3. g Chronic stress causes Golgi apparatus fragmentation in human motor neurons. Representative images and quantification are shown. Cells were analysed at the end of the 48-hour stress protocol as in a. h Chronic but not repetitive stress results in increased cell death in SH-SY5Y cultures. Cell viability was measured on Incucyte using a caspase-3/7 dye. Data were normalised to the 0-h time-point. N=3; ****p<0.0001, one-way ANOVA with Tukey’s post-hoc test. i Chronic but not repetitive stress results in increased cell death in human motor neuron cultures. Cell viability was measured on Incucyte using a caspase-3/7 dye. Data were normalised to the 0-h time-point. N=4; ****p<0.0001, one-way ANOVA with Tukey’s post-hoc test.

Article Snippet: Viability and proliferation analysis were performed on Incucyte® S3 Live-Cell Analysis System (Sartorius), by recording cell density in the bright-field (Phase Object Count) and apoptosis rate using the Incucyte® Caspase-3/7 Green Dye (Green Object Count).

Techniques: Western Blot, Immunostaining, MANN-WHITNEY, Pulse Chase

Journal: bioRxiv

Article Title: In vivo isogenic modelling unveils a TP53 -mediated relapse phenotype in T-cell acute lymphoblastic leukemia

doi: 10.64898/2026.01.19.700255

Figure Lengend Snippet: A. Kaplan-Meier survival curves of PDX mice engrafted with primary isogenic Dx-Rel leukemic cells from 4 T-ALL patients (TALL11, TALL30, TALL42 and TALL43). N=4-5 recipient mice injected at equivalent cell doses per sample. B-C. Parallel serial transplantations of paired Dx-Rel T-ALL PDX samples. Kaplan-Meier survival curves of mice injected with 3 cell doses (10 6 , 10 5 and 10 4 cells) of isogenic Dx-Rel PDX pairs (n = 4 TALL cases) raised from primary ( B ) and secondary ( C ) xenografts. D. LIC frequencies of isogenic Dx-Rel PDX sample pairs calculated from in vivo limiting dilutions. E-F. Real-time in vitro cell proliferation and apoptosis of leukemic cells from isogenic Dx-Rel PDX pairs, measured with the Incucyte® Live-Cell Analysis System. Proliferation was quantified by measuring the proportion of phase objects confluence over time, without labels (E) , and apoptosis by the quantification of green fluorescently labelled nuclei after activated caspase-3/7 cleavage of inert Incucyte® Caspase-3/7 dyes (F) . Statistical data in this figure are represented as mean ± s.d. Significance is indicated by P values; A-C: Log-rank test (Mantel-Cox); D: overall tests for differences using ELDA software; E-F: two-way Anova test. Number of recipient mice and median survival are indicated into brackets on Kaplan-Meier survival curves. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001.

Article Snippet: Leukemic cell apoptosis was detected using the IncuCyte® Caspase-3/7 green reagent for apoptosis ( Sartorius ).

Techniques: Injection, In Vivo, In Vitro, Cell Analysis, Software